The effect of the antioxidant drug “U-74389G” on sodium levels during ischemia reperfusion injury in rats


Background: The aim of this experimental study was to examine the effect of the antioxidant drug “U-74389G” testing, on rat model and particularly in an ischemia reperfusion (IR) protocol. The beneficial effect or non-effectiveness of that molecule was studied biochemically using mean blood sodium levels.

Material and methods: 40 rats of mean weight 231.875 g were used in the study. Sodium levels were measured 60 min after reperfusion (groups A and C) and 120 min (groups B and D) after reperfusion with administration of the drug “U-74389G” in groups C and D.

Results: “U-74389G” administration non-significantly increased the sodium levels by 0.25 mmol/l (-1.479487 mmol/l-1.979487 mmol/l, p=0.7714). This finding was in accordance with the results of paired t-test (p=0.7714). Reperfusion time significantly decreased the sodium levels by 1.85 mmol/l (-3.471346 mmol/l-0.2286544 mmol/l, p=0.0264), also in accordance with paired t-test (p=0.0088). However, “U-74389G” administration and reperfusion time together non-significantly decreased the sodium levels by 0.4636364 mmol/l (-1.496569 mmol/l-0.5692967 mmol/l, p=0.3693).

Conclusions: “U-74389G” administrations as well its interaction with reperfusion time have non-significant alteration short-term effects on sodium. Perhaps, longer experiment times may reveal any possible significant effect of “U-74389G” on blood sodium levels.